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In-vitro drug release and stability assessment of tailored levan–chitosan biocomposite hydrogel

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Abstract

An oxidized levan–chitosan (OLC) cross-linked hydrogel, a 3D network polymeric material, was prepared. Spectroscopic techniques confirmed the oxidation of levan and imine bond formation during cross-linking mechanism. The gelation time for OLC was less than 10 min and the gel exhibited viscoelastic properties with highly interconnected porous structures. The obtained OLC-gel exhibited better thermal stability compared to pristine levan. It was found that the OLC exhibited pH-dependent swelling behavior. The swelling ratio of OLC-gel at three different pH values indicates high hydrophilic nature in the order of pH 2.6 > pH 9.0 > pH 7.4. The hemolytic activity (3%) shown by the hydrogel was found to be within the clinical acceptance level (< 5%). OLC-gel loaded with turmeric oleoresin with 8.28% curcumin content showed a sustained release rate over 24 h. The kinetic release profile of the OLC-gel followed the Korsmeyer–Peppas model with non-Fickian release transport. The synthesized OLC-gel exhibited good cytocompatibility when tested against L929 fibroblast cells. The formed OLC-gel supported cell adhesion and proliferation of fibroblast cells which could support tissue regeneration at the wound site. The characteristics of OLC-gels suggested that OLC has excellent potential to serve as a wound dressing material.

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Authors declare that the data presented in this paper will be made available upon request.

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Acknowledgements

The first author Mrs. Bhuvaneshwari Veerapandian sincerely thanks CSIR for the financial support through the SRF fellowship (Direct SRF/09/1095(0061)/2020 EMR-I). Dr Thirupathi Kumara Raja Selvaraj shows his sincere thanks to SERB-SRG for the financial support (SRG/2021/00562). All the authors sincerely thank the Department of Science & Technology and SASTRA Deemed University for support through the FIST program (Grant: SR/FST/ET-1/2020/614(C)).

Funding

The study was supported by Council of Scientific & Industrial Research, SRF/09/1095(0061)/2020 EMR-I, Bhuvaneshwari Veerapandian, Science and Engineering Research Board, SRG/2021/00562, Thirupathi Kumara Raja, and Department of Science and Technology, Government of India, India, SR/FST/ET-1/2020/614(C), Ponnusami Venkatachalam.

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Author notes

  1. Saravanan Ramiah Shanmugam

    Present address: Department of Biosystems Engineering, Auburn University, Auburn, AL, 36849, USA

Authors and Affiliations

  1. Biomass Conversion and Bioproducts Laboratory, Center for Bioenergy, School of Chemical & Biotechnology, SASTRA Deemed University, Thirumalaisamudram, Tamil Nadu, India

    Bhuvaneshwari Veerapandian, Thirupathi Kumara Raja Selvaraj, Saravanan Ramiah Shanmugam & Ponnusami Venkatachalam

  2. Cardiomyocyte Toxicity and Oncology Research Laboratory, School of Chemical & Biotechnology, SASTRA Deemed University, Thirumalaisamudram, Tamil Nadu, India

    Kartik Kumar Sarwareddy & Krishna Priya Mani

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  1. Bhuvaneshwari Veerapandian
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Correspondence to Ponnusami Venkatachalam.

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Veerapandian, B., Selvaraj, T.K., Shanmugam, S.R. et al. In-vitro drug release and stability assessment of tailored levan–chitosan biocomposite hydrogel. Iran Polym J 33, 11–23 (2024). https://doi.org/10.1007/s13726-023-01229-x

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